36502019赛默飞10µl 进样针 FN 80mm T

Single Primer ("Semi-Random") P

Reamplification 40 µL H2O 10 µL 10x M-PCRB 10 µL 4 dNTPs @ 2 mM each 10 µL Pn primer @ 5 µM 10 µL Po primer @ 0.2 µM 10 µL DNA from part 1 10 µL "T/TS" @ 0.4 u Taq/µL --------- 100 µL Load glass capillaries and amplify: 30"x94°C 30

DNA Sequencing

: G, A, T, C). Prewarm at 37℃ in an incubator. To each annealed DNA sample (Step #2), add the following: 3.0 µL 1 x Labeling mix (dilute 5 x stock with water) 1.0 µL [35 S]dATP (1000 Ci / mmol). Allow to stand at room temperature until used

DNA序列分析（DNA sequencing)

Thermo Sequenase I DNA polymerase (10 U/ul) 1.0 µL Distilled Water (to a final volume of 27 µL) ____ µL