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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
of an appropiate size (400ml is ok). Place the beaker into the vacuum jar. Degass the solution by drawing vacuum until bubles form. Place a paper towel under the beaker to avoid that the beaker gets stuck in the bottom of the vacuum jar. 6. Sprinkle the plants
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
Nalgene jar with cover (Thermo Scientific, cat. no. 5352‐0004) Sterile 1‐ml syringe and 23‐G needle
Synchronizing Protein Transport in the Secretory Pathway
or transported to their target compartments, newly synthesized proteins leave the endoplasmic reticulum to reach the Golgi apparatus, where they are processed and sorted toward their final destinations along the secretory pathway. It is now clear that many Golgi
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