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- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311454169
中文名称：
英文名称：X10 Kanamycin sulfate Supplement freeze dried 10
货期：热销品现货，其他请咨询
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文献和实验Polyclonal and Monoclonal Antibodies
of IMDM add: 0.5 ml 1000X transferrin; 0.5 ml 1000X 2- Mercaptoethanol; 10 ml 50X HT; 5 ml 100X Kanamycin Sulfate; 75 ml FCS ( final 15% ) for fox-NY: IMDM or RPMI; 10 % FCS; 1X AT supplement; transferrin; Kanamycin for J774A.1: same medium
The production of monoclonal antibody by hybridoma
cell culture plates. Reagents: 1-3 x 10 8-9 immune spleen cells 1-6 x10 7-8 myeloma cells in log phase of growth Complete Media No Sera (CMNS) for washing of the myeloma and spleen cells. Hybridoma medium CM - HAT
contained in a 1.5 ml microcentrifuge tube, invert to mix, and incubate in an ice-water bath for at least 10 minutes. It is possible to place the sample at -20℃ overnight at this stage.2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15 minutes
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