赛默飞世尔Thermo Fisher X12 BOTTLE,

ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELS

cut molecular weight standards on the gel. 7.5% Mini (10 x 8.2 cm) Polyacrylamide Gels 10 ml 7.5% Polyacrylamide Gel Solution 65 µl 10% (w/v) APS 12.5 µl TEMED SEQUENCING GELS (8% Polyacrylamide, 8 M Urea

原创：western protocol

. Sponge - filter paper - gel - membrane - filter paper - sponge 3.  Transfer for 1 hr at 1 amp at 4°C on a stir plate. Bigger proteins might take longer to transfer. For the Mini-Transblot, it's 100 V for 1 hr with the cold pack and prechilled buffer

Methods for DNA isolation

in a microcentrifuge. Decant the supernatant and drain. 9. Add 180 ul 10:1 TE buffer, vortex, and incubate at 55degC for 10 minutes. 10. Add 20 ul 2 M sodium acetate and mix. Add 500 ul of cold 100% ethanol, mix, and centrifuge for 1 minute at 12,000 rpm