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文献和实验Who can help me-Molecular Biology
and incubate 55� ON. - phenol/chloro extraction 1h @ 4℃ under soft rotation. - chloro/isoamyl alcool (24:1) - 1 Vol Isopropanol precipitation. Maybe I use a higher conc of proteinase K than you...I don't know, but I think it is a protein contamination
How to prepare Molecular Biology grade glycogen
Overview Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage
Nucleic acid precipitation-Molecular Biology
Hi! Would anyone out there be able to tell as to why alchohols like isopropanol or ethanol are used to precipitate nucleic acids,and also,why is the precipitation done at low temperatures ramesh -ramesh- ----
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