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货号:T_701116704B
中文名称:杜瓦瓶
英文名称:THERMO-FLASK SS 2 LITER
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文献和实验and to the vacuum. Filter the lysate through the Whatman,changing it often as it gets clogged. Filtrate may now be stored a 4℃ until it is time to run over an affinity column. Note: Don't store for more than a couple of days at 4℃. References K.D.Puri,E.B
-40) 1 liter bttle Stir bar 2 Side-arm flasks Prcedure All wrk must be dne at 4℃. 1.Cllect 50 grams f tnsil tissue.(this shuld yield a large amunt f ICAM-1,LFA-1,and PNAd) Nte 1: Cllecting this much tnsils can take sme time.S it is gd t
Protein Expression and Purification Protocol
. (Have a stock of ampicillin in the 4o C which is 1000x concentrated or 100 mg/ml in sterile distilled water to make things easier). b) Pick a colony or two from your plate using a sterile innoculation loop and add to the LB broth/ampicillin flask. Incubate
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