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16309All-glass vacuum filter holder, 47/50mm
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文献和实验Preparation of Nested Deletions
and transfer to neutralizing solution for 5 min consisting of 500 ml of 1.5 M NaCl, 0.5 M Tris, pH 7.4 (150 ml of 5 M NaCl and 250 ml of 1 M Tris, pH 7.4 made up to 500 ml in ddH2O). 9) Rinse filter in two successive 2 x SSC baths (400 ml each; 80 ml of 20 x
cDNA Library Screening Protocol - ZAPII
and transfer to neutralizing solution for 5 min consisting of 500 ml of 1.5 M NaCl, 0.5 M Tris, pH 7.4 (150 ml of 5 M NaCl and 250 ml of 1 M Tris, pH 7.4 made up to 500 ml in ddH2O). 9) Rinse filter in two successive 2 x SSC baths (400 ml each; 80 ml of 20
Preparation of Embryonic Extract
. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml
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