sartorius赛多利斯14062--47----RDNO

ELISA Procedure for Measuring Serum Antibody Titer

. 2. Remove the unbound conjugate by washing with PBS-T. 3. Add 200 µL of a 10 mg/mL so, lution of p-nitrophenyl phosphate in 0.05 M sodium carbonate buffer (pH 9.8, containing 1 mM MgCl2), slowly at room temperature until a yellow color develops

Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum

, 20 mm × 1.2 mm (Waters) Allure Biphenyl column, 5 µm, 50 mm x 2.1 mm (Restek, http://www.restek.com) 4000 QTRAP triple

（共享）ELISA原理与实验方法

is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk