sartorius赛多利斯14062--47------NO

（共享）ELISA原理与实验方法

is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk

Sample Preparation for Microinjection

experiments, the cells may be plated directly on to Eppendorf® CELLocate gridded cover slips placed in the center of a glass slide. Thereby individual cells (55 µm) or cell groups (175 µm) can be located easily after microinjection. Eppendorf Micromanipulator

Sample Preparation for Microinjection

during microinjection, solutions containing the markers should be filtered with a syringe filter (pore size 0.2 µm) whenever possible. Also, before loading the capillary, the sample should be centrifuged for 15 minutes at 4°C (10,000 x g). Peptides