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999
- 供应商：
北京泽平
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1年
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EA
货号：T_70103-500-757
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英文名称：STD. SUB COMB 1MM X 10 WELL
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文献和实验Clone Genes From a Phage Librar
MgSO4 to 10mM and pour into 100 ml bottles for future microwaving. (Top agar and other large-scale phage growth solutions do not have maltose because it is not needed when infecting cells with a vast local excess of phage, and you don't want to lose
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
, 6.1g anhydrous Na2HPO4, 2g KH2PO4, H2O to 1 liter. Autoclave and store at room temperature.- 10x Taq Buffer: 500 mM KCl, 100 mM Tris-HCl (pH 9.0 at 25oC), 1% Triton X-100. - 20x SSC: 3 M NaCl, 0.3 M Na3Citrate-2H20. Store at room temperature.-
in a 1.5 ml microcentrifuge tube, invert to mix, and incubate in an ice-water bath for at least 10 minutes. It is possible to place the sample at -20degC overnight at this stage. 2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15
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