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货号:T_701AA33323AP
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英文名称:HYDROGEN PEROXIDE ACS 500ML
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文献和实验Large-scale yeast transformation
, handy for using 12 channel pipettor to plate. (Fisher #12-565-296; $34/60). These can be washed with water, soaked in 3% hydrogen peroxide, and reused. 8 or 12 channel manifold pipettor. Large orifice tips for mixing PEG. Reservoirs
Monoclonal Antibody Production Protocol
1 - Substrate = ABTS - 100X (from Zymed 00-2001)1 - HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dehydrate + 50 ml H2O, pH 4.2 ). - Hydrogen peroxide 30% ( 1000X ) Protocol: ABSORBTION OF ANTIGEN Dilute Ag to 10 ug/ml in PBS
Synthesis and Probing of Membrane-bound Peptide Arrays
above, horseradish peroxidase-labeled detection agents require the use of hydrogen peroxide, which gradually destroys the peptides on the array. More sensitive detection can be achieved with a chemiluminescent substrate of AP (e.g., Immun-Star). If such a substrate
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