赛默飞世尔Thermo Fisher Comb, 1.0mm

Loss of Heterozygosity

with a pipette. Insert the teeth of the comb approximately 1 mm into the gel. Pre-heat the gel at 1700 volts for 15-20 minutes. F: Gel Loading Remove the samples from the freezer. Denature the samples in a thermal cycler at 95°C for 5 mins. Remove

Agarose Gel Electrophoresis

in gel tray about 1 inch from one end of the tray and position the comb vertically such that the teeth are about 1-2 mm above the surface of the tray Pour 50EC gel solution into tray to a depth of about 5 mm. Allow gel to solidify about 20 minutes

The ribonuclease protection assay (RPA)

for 5 to 10 minutes (do not dry in a vacuum evaporator centrifuge). Add 50 µl of hybridization buffer and solubilize the pellet by gently vortexing for 20 seconds and quick spin on microfuge. 8. Quantitate duplicate 1 µl samples