赛默飞世尔Thermo Fisher Comb Fisher

SDS-PAGE

polymerizes (10 min) pour off the overlay, and rinse out the well with dH2O. Then pour all the water out, and dry the well by shoving in a paper towel or kimwipes (carefull not to touch the gel!) Insert the comb.5. Stacking gel : enough for two 0.8 mm

v468 chapter 5 糖原合成酶激酶-3 的抑制

, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v

Separation of Histone Variants and Post‐Translationally Modified Isoforms by Triton/Acetic Acid/Urea Polyacrylamide Gel Electrophoresis

1.5‐mm‐thick 16‐well gel comb Disposable syringes with 23‐G needles Disposable syringe with 18‐G