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文献和实验Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
(Nunc 177402) Transfer pipettes, sterile disposable (Fisher) Tubes, sterile conical (15- and 50-mL) Tubes, sterile microcentrifuge (1.5-mL) Water bath preset to 37°C METHOD Dissection 1. Carefully remove
triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886
) was from Fisher and triethylamine (TEA, Cat. No. T-0886) was from Sigma. Fluorophores were purchased from Amersham (Cy3, Cat. No. PA23001; Cy3.5, Cat. No. PA23501; Cy5, Cat. No. PA25001) and Molecular Probes (Oregon Green 488, Cat. No. O-6147; Alexa Fluor 488
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