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文献和实验Preparation of Embryonic Extract
. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml
Preparation of Embryonic Extract
. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml conical disposable centrifuge tubes
【转贴】immunofluorescence staining
, place the slides in a holder, drawer, or other means of protecting from light.When finished mounting, excess mounting media will spread out from under the coverslips. Suction this off. The suctioning may need to be repeated several times - if you don't
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