赛默飞世尔Thermo Fisher X1920 PIPET

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make

E-Z 96 X-press Plasmid Vacuum Protocol

the vacuum manifold by place a new 300 μl 96-well collection plate (provided) inside the base of manifold. If Omega manifold (Vac-03) is used in this procedure, a used E-Z 96 X-press Plate or a 800 μl plate should be placed under the 300 μl

DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)

of the reagent per 2 x 106 of cultured cells.    2) Cells Grown in Monolayer Lyse cells directly in a culture dish by adding 300 μL of Cell Lysis Buffer per 2 x 106 of cultured cells directly into each well of multiwell cell culture plate or flask