- 询价
- Roche
- 11745832910
- 进口
- 2025年07月15日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 供应商：
广州威佳科技
- 英文名：
DIG-High Prime DNA Labeling
- 库存：
大量
- 规格：
kit
比普通DIG随机引物法标记效率高,操作简单,可用于Southern,Northern和斑点杂交以及菌落和噬菌体筛选.一个kit可用于12次标记反应和24次检测反应.组成:5×Dig-高效标记试剂;未标记对照DNA,PBR328;DNA衡释液;抗-Dig-碱性磷酸酶复合物;NBT/BCIP储存液;10x封闭液;Dig定量试纸条;Dig对照试纸条.-20℃保存.
Catalog # Product name / Packsize
11745832910
(1745832) DIG-High Prime DNA Labeling and Detection Starter Kit I
1 kit (12 labeling reactions and 24 detection reactions)
Application
DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits. Plasmid, cosmid, and lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 μg of template DNA, which may be linear or supercoiled.
Product Description
Sensitivity and specificity: The standard assay uses 1 μg of linearized pBR328. It is usual to expect 0.8 μg labeled DNA after 1 hour of labeling, and 2.3 μg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 μg of digested placenta DNA.
Contents
DIG-High Prime, 5x conc., 50 μl
DIG-labeled Control DNA, 20 μl, (5 μg/ml), pBR328 (linearized with Bam HI)
DNA Dilution Buffer, 3 x 1 ml
Anti-digoxigenin-AP Conjugate, 100 μl
NBT/BCIP Stock Solution, conc., 6 x 1 ml
Blocking Solution, 10x conc., 4 x 100 ml
DIG Easy Hyb Granules, 4 x 100 ml
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文献和实验标记DNA探针 如果您有足够量的高纯度模板，建议您采用随机引物标记方法的DIG High Prime DNA Labeling and Detection Starter Kit I 和II。这两个试剂盒包含了标记、封闭、杂交和检测所需要的所有试剂，是真正意义上的一站式服务的试剂盒。二者之间的差别在于检测方法的不同：Starter Kit
, or Fluorochromes Procedures for Labeling DNA, RNA, and Oligonucleotides with DIG, Biotin, or Fluorochromes 41 Random primed labeling of ds DNA with DIG-, Biotin- or Fluorescein-High Prime reaction mix 41 PCR labeling of ds DNA with the PCR DIG Probe Synthesis Kit
【建议】Southern和Northern中的奇怪问题？请大家讨论。
的DIG Northern Starter Kit，没有用他的显色底物，而是用的DIG High Prime DNA Labeling and Detection Starter Kit I中的NBT/BCIP底物，因为我没有显影液和定影液，所以没有用CDP-Star。还有为什么在边缘的条带跑的快，是不是MOPS电泳缓冲液的原因，我在整个电泳过程中没有换过缓冲液，电泳大约2个小时左右，没40分钟停止电泳，拿出胶后搅一搅缓冲液。 第二张图是Southern杂交，用的是DIG High Prime
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