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文献和实验plunger is inserted. Tighten and seal the adjuster plunger fittings, leaving the inlet tube in the buffer reservoir. Attach the outlet tubing to the peristaltic pump, and set the pump to run at 0.25 ml/min to allow the column to pack properly
Porcine brain tubulin preparation
and seal the adjuster plunger fittings, leaving the inlet tube in the buffer reservoir. 15. Attach the outlet tubing to the peristaltic pump, and set the pump to run at 0.25 ml/min to allow the column to pack properly for the next ~48hrs while MT
Fastfilter Plasmid Maxi Kit Spin Protocol
2. Pellet up to 100-200 ml bacteria in appropriate vessels by centrifugation at 3,500-5,000 × g for 10 min at room temperature. 3. Decant or aspirate medium and discard. To ensure that all traces of the medium are removed, use a clean paper towel
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