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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Cynomolgus / Rhesus GPRC5D Protein (Nanodisc, His & Flag Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg/100 µg/500 µg
| 规格: | 20 µg | 产品价格: | ¥15570.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥54570.0 |
| 规格: | 500 µg | 产品价格: | ¥109170.0 |
蛋白名称:Cynomolgus / Rhesus GPRC5D Protein (Nanodisc, His & Flag Tag)
蛋白构建:A DNA sequence encoding the Cynomolgus / Rhesus GPRC5D (A0A2K5W6I7) (Met1-Ser268) was expressed and linked by a Fusion Protein, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主:HEK293 Cells
蛋白纯度:≥ 80 % as determined by SDS-PAGE.
蛋白活性:Immobilized Recombinant Rhesus GPRC5D-Nanodisc Protein (His & Flag Tag) (Cat: 91022-C18H-NA) at 5 μg/mL (100 μL/well) can bind Anti-Rhesus GPRC5D Antibody, the EC50 is 17.5-52.5 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant Cynomolgus / Rhesus GPRC5D consists of 286 amino acids and predicts a molecular mass of 32.95 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 31.7 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号:A0A2K5W6I7
蛋白氨基酸序列:Met1-Ser268
蛋白标签:N-Flag & C-His
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文献和实验科研人必看:多肽、蛋白质、重组蛋白分不清? 应用场景有哪些?定制如何选?
(Anti-FLAG 亲和凝胶纯化,温和洗脱,保持活性)、Strep 标签 (Strep-Tactin 亲和纯化,高纯度需求优选) 促溶标签:MBP、SUMO、GST 等,可显著提高难表达蛋白的可溶性 功能标签:Avi 标签 (常用于定点生物素化)、GFP (荧光标记,用于蛋白定位示踪) 无标签蛋白:如需天然构象或去除标签干扰,MCE 也支持无标签蛋白制备 『定制需求』 膜蛋白:拥有三大膜蛋白表达技术平台,包括去垢剂平台 (基于哺乳动物细胞表达系统)、纳米盘 (Nanodisc) 技术平台和病毒样
北京义翘神州生物技术有限公司(Sino Biological Inc.)--致力于为全球生命科学研究人员提供高质量的重组蛋白、抗体等科研工具试剂。 • 国际一流的团队:4名中央"千人计划",300人科研团队 • 一流的技术平台:蛋白表达大规模生产,鼠单抗与兔单抗技术 • 国内最大蛋白、抗体库:2000多种蛋白,1500多种抗体 • 抗体检测限比国际知名品牌抗体低10~160倍 • 3000多家全球客户:哈佛医学院、斯坦
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