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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human SLC34A2 Protein (VLP, Full-Length, His-Strep Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg/100 µg/500 µg
| 规格: | 20 µg | 产品价格: | ¥4980.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥10480.0 |
| 规格: | 500 µg | 产品价格: | ¥36880.0 |
蛋白名称:Human SLC34A2 Protein (VLP, Full-Length, His-Strep Tag)
蛋白构建:A DNA sequence encoding the Human SLC34A2-VLP (Full Length) (O95436-2) (Met1-Leu689) was expressed with a C-terminal polyhistidine tag followed by an Strep Tag.
表达宿主:HEK293 Cells
蛋白活性:1. Immobilized Recombinant Human SLC34A2-VLP Protein (Cat: 18959-H57H) at 5 μg/mL (100 μL/well) can bind Anti- SLC34A2 Monoclonal Antibody, Human IgG1 (lifastuzumab), the EC50 is 3-12 ng/mL. 2. Immobilized Recombinant Human SLC34A2-VLP Protein (Cat: 18959-H57H) at 5 μg/mL (100 μL/well) can bind Anti- SLC34A2 Monoclonal Antibody, Human IgG1 (Upifitamab), the EC50 is 4-12 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Met
蛋白分子量:The recombinant Human SLC34A2-VLP consists of 714 amino acids and predicts a molecular mass of 78.55 kDa.
蛋白NP号:O95436-2
蛋白氨基酸序列:Met1-Leu689
蛋白标签:C-His-Strep
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文献和实验Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
a methodological workflow based on an SF?TAP tag comprising a doublet Strep?tag II and a FLAG moiety optimized for rapid as well as efficient tandem affinity purification of native proteins and protein complexes in higher eukaryotic cells. Depending
a procedure for the isolation of nondenatured, recombinant human AR with more than 95% purity using four-step chromatography with milligram yields (see Notes 1 -Notes 4 ). Purified AR may be used in physical and biochemical studies, such as monoclonal antibody
Purification of Human Multiprotein Complexes using OneSTrEP Technology
sufficient for visualising single protein bands by Coomassie Blue staining (Figure 1). The Strep -tag®II (SAWRHPQFGG) and its “double” sister, the One-STrEP-tag (tandem arrangement of Strep -tag®II, here called OneStrep), are reasonably small protein
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