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文献和实验2 for 2 h enhancedglycogen synthase activity twofold and increased cytoplasmicb-catenin levels (18) . 1.1.2. Small Molecule Inhibitors of GSK-3 Small molecule inhibitors of GSK-3 consist of two groups: ATPcompetitive and ATP noncompetitive. Among ATP
Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes
measures the frequency of t(14;18) translocations that result in the dysfunctional regulation of the antiapoptotic gene BCL-2 . The other assay measures the frequency of a deletion caused by illegitimate V(D)J recombination in the X-linked HPRT gene.
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
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