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- 2025年12月25日
- 仅仅用于动物模型体内研究
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仅仅用于动物模型体内研究
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文献和实验, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v
V294.PART II，Chapter 5 细胞粘附实验（英文版）
7.4) and incubate at room temperature for 1 h. 3. Remove coating solution and add 1 mL of 1% w/v BSA in PBS. Incubate at roomtemperature for 1 h. 4. Remove BSA solution. Wash twice with PBS. 3.2.1.3. PREPARATION OF PURIFIED SUBSTRATE ON GLASS COVER
A Dock and Lock Approach to Preparation of Targeted Liposomes
We developed a strategy for covalent coupling of targeting proteins to liposomes decorated with a standard adapter protein. This strategy is based on “dock and lock” the interactions between two mutated fragments of human RNase I, a 1–15-aa
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