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- 2025年11月09日
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11106-293------NCA Membrane_ 0.45µ_ 293mm_ 100pk
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文献和实验Preparation of Brain Membrane Fractions
Preparation of Brain Membrane Fractions for Western Blot AnalysisAll the procedures should be done at 4ºC using precooled reagents. For rat samples, immediately remove brain from the cranium into ice cold dissection buffer (50 mM Tris-acetate (TA
Transient transfection into 293T cells
Purpose Transient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line which is transformed by adenovirus E1
Membrane Transfer and Crosslinking for RNA
The composition of the transfer buffer is usually a 5X SSC/10 mM NaOH solution. These mildly alkaline conditions shear the RNA into smaller fragments and denature it as it is deposited onto the membrane. A brief protocol for assembly (see Figure 1
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