Sartorius赛多利斯VN01H42ETOVivacon

TRICINE GELS Protocol (For Low MW proteins (

Solution   Methanol CP  500ml 50% Acetic Acid CP 100ml 10% H2O 400ml  

Protocol for competitive RT-PCR

volume of a 10x PCR standard buffer (15 mM MgCl2; 100 mM Tris /HCl, pH 8.3; 500 mM KCl) are added to a total volume of 50 µl. The PCR is run in the thermal cycler GeneAmp 9600 (Perkin Elmer). The PCR products are then separated on a 1.5% agarose gel

Invitrogen RT-PCR 新工具

液以提高反应灵敏度和产量。性能卓越的混合物能够从低至0.1pg的总RNA中检测到看家基因（图1）和从长达12kb的靶标中高产量地合成cDNA，以进行多种RT-PCR应用，包括基因检测和全长基因克隆。 图1-SuperScript™ III First--Strand Synthesis SuperMix提供卓越的灵敏度 使用SuperScript™ III First--Strand Synthesis SuperMix，0.1pg到100