sartorius赛多利斯14076--47------NT

（共享）ELISA原理与实验方法

)9 Incubate 30 min at 37°C.10 Rinse 3x with 0.05% Tween 80 in 0.01 M PBS, pH 7.4.11 Rinse 3x with HPLC water.12 Add 50 µl of color development reagent(1 tablet of p-nitrophenyl reagent in 5 ml of 10% diethanolamine, pH 9.8) (mix well)13 Incubate 30 min at 37°C

Sample Preparation for Microinjection

experiments, the cells may be plated directly on to Eppendorf® CELLocate gridded cover slips placed in the center of a glass slide. Thereby individual cells (55 µm) or cell groups (175 µm) can be located easily after microinjection. Eppendorf Micromanipulator

Sample Preparation for Microinjection

during microinjection, solutions containing the markers should be filtered with a syringe filter (pore size 0.2 µm) whenever possible. Also, before loading the capillary, the sample should be centrifuged for 15 minutes at 4°C (10,000 x g). Peptides