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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
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EA
货号:T_70312570688
中文名称:
英文名称:MASS FLOW CONTROLLER 50L/MIN
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文献和实验μM calyculin A, 2 mM PMSF, and 10 μg/ml aprotinin). 4) Lyse for 20 min on ice and clarify lysates by centrifugation at 16,000 x g for 10 min at 4 oC 5) Preclear lysates with 30 μl of a 1:1 slurry of protein G-sepharose (Fast-Flow; Pharmacia
Measurement of Arginine Metabolites: Regulators of Nitric Oxide Metabolism
) L‐arginine, L‐citrulline, proline, L‐ornithine, ADMA, SDMA, and NMMA 0.1 M HCl (ACS reagent grade; Fisher)
are performed using a 5‐µm Supelcosil LC‐18S column (25 cm × 4.6 mm) according to the following conditions: starting from 0.1 M triethylammonium acetate, pH 7.0, a linear gradient of 2.5% MeCN/min is pumped at a flow rate of 1 mL/min for 40 min. Peak heights
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