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文献和实验, but in only one are the chromosomes separated sufficiently for counting. That spread is magnified in the right-hand image showing 20 chromosomes, the proper diploid karyotype of X. tropicalis . Figure 2: Appearance of X. tropicalis inbred (Nigerian) line
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
RNA Purification Protocol for 10-20mg of Animal Tissue
) or glycogen (Cat # AC122) to the 100ìl isopropanol. 7. Gently mix the solution by inverting the tube 30-40 times. 8. Centrifuge at 13, 000 x g for 5 minutes at room temperature. 9. Pour of the supernatant and drain the tube briefly on a clean
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