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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
, activate AEC buffer using H2 O2 (see Step 25). Anti-CD3 monoclonal antibody (mAb) (Fisher Scientific) Blocking buffer for ELISPOT assay CEF peptide pool (available from the NIH AIDS Research and Reagent Program and other commercial
免疫组化技术-Immunohistochemical Protocols
(mostly these are IgG's), and then an appropriate pre-conjugated anti-IgG molecule to detect the primary antibody. In addition to these conventional immunostainings, a newer method has been introduced with increased sensitivity. This is called the tyramide signal
per immunoprecipitation. You can use less (as little as 50) but the higher amount gives you a good, easily detectable amount of activity. 9.) To the lysate, add 1.5 μg of anti-ERK antibody (Santa Cruz, SC154 (Erk2) or SC93 (Erk1)), and incubate, with rocking
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