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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311769518
中文名称:
英文名称:X4 PCR PLATES ADAPTERS
货期:热销品现货,其他请咨询




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文献和实验PURIFICATION OF PCR PRODUCTS WITH SEPHADEX
. Remove the plates containing the PCR products from the refrigerator. Spin them at 4000 rpm for 1 min. Add samples (should be ~50 � in each well) very carefully (try not to touch the sephadex) to the center of the sephadex unifilter using
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
inactive adapter ligation reaction at 65℃ for 5 minutes. Remove excess adapters and enzymes by spin chromatography using QIAquick PCR Purification Kit #28106 pg. 18 of the QIAquick Spin Handbook. Elute 1X in 30µl preheated EB, 1X in 30µl preheated water
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