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- 2025年07月08日
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- 库存：
999
- 供应商：
北京泽平
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热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70113-946-12C
中文名称：
英文名称：REFRACT INDX SET A-1/2 46LIQ
货期：热销品现货，其他请咨询
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
gauge needle with a thin piece of tygon tubing which I can slide to the bottom of the tube). Spin at 12,700 rpm for 15 at 4 deg.C for 15'. As soon as spin is started, process the next set of plates through steps 4-7. Aspirate supe till approx
: 12,500 rpm for 15' at 4 deg.C in HB-4. Post fix in methanol (-20 deg.C) for 5' and rehydrate and do 5051 + antitubulin followed by anti-mouse, anti-human secondaries. Assess peak by concentration of double staining dots, pool and freeze in liq N2
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