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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310099910
中文名称:
英文名称:X4 O-ring for 90mm holder
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文献和实验Preparation of Embryonic Extract
. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml
Immunohistochemistry on fixed, paraffin-embedded sections
Indirect immunohistochemistry with avidin-biotin peroxidase(e.g rabbit anti antigen X, goat anti-rabbit biotin, avidin HRP) 1- Wash twice in TBS-T. 5 min / wash. 2- Briefly blot the slides without letting them dry
In Situ HYBRIDIZATION TO TISSUE SECTIONS
.C.T. (Tissue-Tek O.C.T. Compound) Paraffin (Paraplast Plus, Fisher) 8% Paraformaldehyde (Polysciences E.M. Grade 0380): Prepare fresh in 1X PBS. Dissolve 8g paraformaldehyde in 100ml buffer at 65-80 degrees C. Add 1-2 drops 50% KOH
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