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北京泽平
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货号:T_70113-880-404
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英文名称:2-1.3L/MIN LIQ TRANSFER PUMP
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文献和实验Northern Blotting方法(Howell Lab)
for at least 30 min before quantitation. Pipet up and down several times before taking 2 µl into 998 µl water. Read at 260 nm. RNA concentration (µg/µl) = OD x 500 x 0.04 µg/µl = OD x 20 Formaldehyde gel Boil 2 g agarose in 144 ml
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
as stage X, nearly 24 h earlier in development than most electroporation protocols. Compared to in ovo electroporation, the ex ovo method allows easier embryo orientation (the posterior half of the embryo is darker than the anterior half
) to remove residual bleach. 4. For small collections ( m L 40% formaldehyde. For large collections (>5 mL of settled embryos), transfer embryos to a 1 L bottle containing 300 mL heptane, 90 mL PBS and 10 mL 40% formaldehyde. Shake for 20 min. 5.
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