赛默飞世尔Thermo Fisher 14L CIRCULA
TOR DIG WATER+OIL BATH temperature controlled 230V 50-60Hz 1250W 360mm x 货号：T_70311716587- ￥1200 - 24000
- Thermo Fisher
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- T_70311716587
- 2025年07月10日
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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311716587
中文名称：
英文名称：14L CIRCULATOR DIG WATER+OIL BATH temperature controlled 230V 50-60Hz 1250W 360mm x
货期：热销品现货，其他请咨询
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文献和实验In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
% hybridization buffer (HB) and incubate 5 min at 48°C (in water bath). 13) Pre-hybridize in 400 ul HB for 1 hr at 48°. 14) Single-stranded DNA probes are made by asymmetric PCR using a single primer (see Patel and Goodman protocol distributed by G
8. 3M sodium acetate. 9. Cold 100% ethanol. 10. Cold 70% ethanol. 2.2 Embryo Collection and Fixation 1. Chlorine bleach solution diluted 1:1 with water. 2. 20 mL glass scintillation vials (Fisher Scientific
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
of hybridization buffer and PTw, followed by a 10 min incubation in undiluted hybridization buffer. (These incubations are done at room temperature). During this time, a separate 150 ml aliquot of hybridization buffer is heated in a boiling water bath for 10 min
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