赛默飞世尔Thermo Fisher COL,ACCUCOR

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

of 20 mM HEPES Buffer in 0.9% saline solution. In a separate conical tube, create a T solution by adding 17 μl of the thrombin stock, and 1.3 μl of 2 N Ca solution to 135 μl of DMEM. See Table 1.    2)      In a third conical tube, prepare

（共享）免疫组织化学染色FAQ

in perfusion fixation ** Michel's fluid for transporting cells or specimens ** Microwave ovens: Advice for new users ** Paraformaldehyde: why won't it dissolve? ** Saccomano's fixative ** Zinc-containing fixatives: What has been published? ** Alternatives

Stripping Western Blots

units/ml). Now, I don’t know what a ‘unit’ of phalloidin is, but this stock is effectively 100X. 2) For DAPI (D-1306 from Molecular Probes), we dilute the supplied 10mg in 9.5mls of H2O, giving a stock of 3mM. This is ~5x less concentrated