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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70315152878
中文名称:
英文名称:Screw Cap Tube, 12 x 80mm
货期:热销品现货,其他请咨询




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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column (BIO-RAD cat. #732-6008)Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
In vitro transcription reaction
-HCl, pH 7.5; 0.1 M NaCl; 10 mM EDTA). 2- Load transcription reaction on the column. Place screw cap tube to collect the radioactive probe. Pipette on top of the column 70 ml more STE and collect into the same tube. Mix and measure volume (~110-130 ml
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