赛默飞世尔Thermo Fisher OPTIMA-1 ID

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

2.33 ml 2.33 ml 2.33 ml 2.33 ml Gel A/B 1.74 ml 2.05 ml 2.21 ml 2.53 ml 2.84 ml 3.16 ml Gel A 188 µl 223 µl 240 µl 275 µl 309 µl 343 µl TEMED 2.55 µl 2.29 µl 2.16 µl 1.91 µl 1.65 µl 1.4 µl 10% APS 25.5 µl 22.9 µl 21.6 µl 19.1 µl 16.5 µl 14 µl

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge

Southern Hybridization Protocols

do not have the reproducibility and robustness of the former Boehringer) are used in our laboratory.Place 25ng (1 m l) of template DNA into a microcentrifuge tube. Add in 5 m l of primers and appropriate volume of 50 m l in the final reaction. Denature by heating to 95-100 ℃