赛默飞世尔Thermo Fisher SYNCRONIS S

DNA Extraction Protocols Using Silica

sporadically).6. Spin out the silica by centrifugation. Save the supernatant wrapped in aluminum foil or transfer to a brown bottle (stable 1 month at room temperature).D. Washing Ethanol70% EtOH supplemented with 10 mM NaCl (e.g., for 500 mL add: 368.4 mL 95

Construction and Characterization of Adenovirus Vectors

stock solution with H2 O and sterilize by autoclaving for 45 min at 121°C. Complete medium Dialysis buffer (10 mM Tris-HCl, pH 8.0) Sterilize by filtration through a 0.2-µm filter. DNase I (Ad) Ethidium bromide

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);