赛默飞世尔Thermo Fisher X125 Headsp
ace vial Chromacol screw top 22mm x 45mm 10mL (pack of 125) 货号：T_70311981523- ￥70 - 1400
- Thermo Fisher
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- T_70311981523
- 2025年07月12日
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311981523
中文名称：
英文名称：X125 Headspace vial Chromacol screw top 22mm x 45mm 10mL (pack of 125)
货期：热销品现货，其他请咨询
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
In Situ HYBRIDIZATION TO TISSUE SECTIONS
. The devittellinized embryos will sink to the bottom. Transfer the embryos to a vial with a wide bore pipet tip. Wash 3X with 90% MeOH/EGTA. Rehydrate embryos (0-2 and 1-3 hr embryos need not be rehydrated through paraformaldehyde, just leave
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