赛默飞世尔Thermo Fisher POT HYDROXI

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

, and the reaction is carried out at 70°C. Subsequently, workup, isolation, and purification lead to the requisite O 6 ?alkyl or O 6 ?aryl ethers in good to excellent yields. Curr. Protoc. Nucleic Acid Chem. 49:1.26.1?1.26.16. © 2012 by John Wiley & Sons

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

thymidine 5′‐triphosphate (TTP, 8t O ). The crude sample can be analyzed on a Welchrom C18 (or Ultisil C18) reversed‐phase column (4.6 × 250 mm) measured at 260 nm at a flow of 1.0 mL/min using a linear gradient of 0% to 40% buffer B in 20 min. Buffer A: 20

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration