赛默飞世尔Thermo Fisher POT HYDROXI

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

L per µL reaction) at 37°C for 1 hr. Reactions were analyzed by 19% polyacrylamide gel electrophoresis. Lanes: (1) 5′‐32 P‐labled primer; (2) primer and all dNTPs, but no Kf−; (3) positive control: primer, template, all commercial dNTPs, and Kf−

Methods for DNA sequencing

template DNA  2 μl  Bst reaction buffer  2 μl  Bst nucleotide extension mix  1 μl  oligonucleotide primer (2.5 ng/μl)  0.5-1 μl [alpha]-32-P-dATP or [alpha]-35-S-dATP  1 μl  diluted Bst polymerase (0.1 U/μl)  q.s.  sterile ddH2O  12 μl [alpha]-32-P

（共享）免疫组织化学染色FAQ

maleimide (= 12.5 g in 1 L of water) (Sigma E3876.) Water to make 1 L Adjust to pH 7.0 with 1 M potassium hydroxide Store in refrigerator. (Cost about $50/25 g in 1994.) Transport medium: Dissolve 55 grams of ammonium sulfate in 100 mL washing