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货号:T_70114-379-332
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英文名称:VIAL SAMPLE PP 160ML PK/10
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文献和实验AML Sample genomic DNA Preparation
% EtOH Phenol: Buffered to pH=8 with Tris. Chloroform: Stabilized with isoamyl alcohol. T.E.: 10 mM Tris pH8, 1 mM EDTA. Note This protocol prepares genomic DNA from cryopreserved leukemia cells. It assumes a starting volume of ~1.8 mL
), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
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