赛默飞世尔Thermo Fisher 100ml量筒 CYL

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

making it possible to use the technique in E. coli and other commonly used bacterial strains (10). In addition, the recombination efficiency was increased 10�100 times (11). The combination of these three enzymes (α, β and γ, or E, T and γ) in one vector

Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period

100. Add 10 ml of 1 M Tris, 109.54 gr of sucrose, 1.01 gr MgC12, adjust pH to 8.0 and finally add 10 ml of Triton X-100 to 800 ml of distilled water, and make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 10 min. Sugars at high

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

7 mL of 1.15X ligation buffer. 19. Add 400 µL of 20% Triton X-100 (to a final concentration of 1%). Incubate with occasional gentle shaking for 1 h at 37°C. 20. Add 50 µL of T4 DNA ligase. Incubate for 4 h at 16°C