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999
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货号：T_701FA10027G
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英文名称：PIPETMAN L FIXED, F300L
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文献和实验Immunohistochemistry on fixed, paraffin-embedded sections
. If one of your antibody is biotin-conjugated, you need at this point to do endogenous biotin blocking . 4- blot the slides without washing and apply the primary antibody (100µl), in a moist chamber, at RT for 1-18 hr. 5- Wash twice in TBS-T. 5 min / wash
A quick RNA mini-prep for Neurospora mycelial cultures
thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed
Staining Procedure for Flow Cytometric Detection of Human Cyclins
No. 3 above. II. Staining 1. Just prior to staining, remove ethanol by centrifugation at 200 x g for 10 min. Aspirate and wash once by resuspending pellet in 30-40 m l of Wash buffer. Centrifuge at 200 x g for 10 min
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