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货号:T_70107-200-130
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英文名称:RESERVOIR 100ML IND BAG 100/CS
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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Northern Blotting方法(Howell Lab)
of buffer is sufficient. If a bag is used, it's about 0.2 ml of buffer per square cm of membrane. It is critical that the mem-brane be wet thoroughly. Boil 200 µg/ml of sheared salmon sperm DNA in a 50 ml conical tube for 5 min and chill
of PBS-CMF and the cells were then deposited by cytocentrifugation (450 rpm x 5 minutes, Shandon cytocentrifuge) onto two glass slides. Viral RNA was digested with ribonucleases A (100 µg/ml) and T1 (10 µg/ml), and the cells were postfixed for two hours
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