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文献和实验Kodak X-OMAT AR film (XAR) (Fisher, 05-728-41) 14 x17 Kodak X-OMAT AR film (XAR) (Fisher, 05-728-42) 8 x 10 and 14 x 17 intensifying screen (Fisher, FB-IS-810 and FB-IS-1417) ART pipette tips (Rainin) Protocol DNA Preparation/Transfer to Membrane
use isoamyl alchohol here). Add 2 volumes (~0.8 ml) 100% EtOH at room temp. Invert and flick as before, and again pick the pellet out with a pipette tip to a tube of ice cold 70% EtOH. The nucleic acid precipitate will be substantially smaller
). Incubate 37° for 1-2 hour. Phenol/CIA extract as before. Chloroform extract (I often don't use isoamyl alchohol here). Add 2 volumes (~0.8 ml) 100% EtOH at room temp. Invert and flick as before, and again pick the pellet out with a pipette tip to a tube
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