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北京泽平
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EA
货号:T_70107-200-129
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英文名称:RESERVOIR 100ML BULK 200/CS
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文献和实验Procedures for In Vitro Production of Bovine Embryos
hood 100 ml pipet tips and pipettor OMM + supplements Mineral oil 60 x 15 petri dishes Plastic pasteur pipet 3-4 bottles of OCM + supplement (warmed @ 38.5°C) 1 L saline + Pen/Strep (warmed @ 38.5°C)Preparation for Oocyte Collection Scalpel Petri dishes
Northern Blotting方法(Howell Lab)
5 ml of scintillation fluid and count. % incorporation = (cpm of washed/cpm of unwashed) x 100. Anything over 10% is usually usable. Using the kit, it's usually 40%. The specific activity (S.A.) of the probe is also important. S
Fusion of Mouse, Rat, or Hamster Cell
the pellet in HAT medium to 5 x 105 cells/ml. Plate 200 ml/well to 96 well plates. Incubate at 37o C, 8-10 CO2 for 5-7 days.
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