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文献和实验Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
Orbital Shaker • Water Bath, VWR Scientific • 500 ml Vented Cap Erlenmymer Flask, • 125 ml Vented Cap Erlenmymer Flask, Corning product • Pro293S-CDM, 1 L (Cambrex Biosciences, Cat 12-765Q) • Penicillin-Streptomycin, Invitrogen • Transfection
triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
of interest) using an endotoxin-free DNA purification kit, e.g., the EndoFree Plasmid Maxi Kit (Qiagen). Store the purified stock in endotoxin-free H2 O. 6. Prepare the plasmid DNA for electroporation by mixing a 5 µg/µL concentrated stock
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