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货号：T_70311969227
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英文名称：X100 FILTER PURADSIC 25 GD25MM GD
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
with phenol/chloroform/isoamyl alcohol (25:24:1), spinning 12000g 10 min at 4°C in a fixed angle rotor. Save aqueous (upper) phase; add gd H2 O if volume is less than 2 ml. 12. Add 0.1 vol 3M NaOAc pH 6.0, mix and add 2.5 vol EtOH, mix. Place at -20°C
A quick RNA mini-prep for Neurospora mycelial cultures
10. Spin at 12000g, 4°C, for 15 min in a fixed angle rotor. Thoroughly resuspend pellet in 4 ml sterile gd (glass distilled) H2 O with pasteur pipette. 11. Extract twice with phenol/chloroform/isoamyl alcohol (25:24:1), spinning 12000g 10 min
experiment.Disslve in 100% EtH) 1 mM Benzamidine 5 mM Idacetamide Whatman Paper Centrifuge Bttles Funnel Squeeze bttle Several Beakers Hmgenizer (Fisher PwerGen 125) 50ml Crning tubes Ice Bucket & Ice Dunce hmgenizer (40ml Pyrex Cat N.7727
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