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文献和实验Intraspinal Transplantation of Mouse and Human Neural Precursor Cells
the preparation and transplantation of human neural precursor cells (hNPCs) and mouse neural precursor cells (mNPCs) into the thoracic region of the mouse spinal cord. The techniques in this unit also describe how to prepare the mouse for surgery by performing
Keywords: human embryonic stem cells; mouse neural stem cells; MTT assay; 96?well plate assays; toxicology GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
lineages. They can be maintained either on a layer of mitotically inactivated human or mouse feeder cells (1) or using mouse or human feeder cell conditioned medium (2) (R&D Systems, Catalog # AR005 or AR007 , respectively). The protocol below has been used
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