赛默飞世尔Thermo Fisher NEEDLE TUBE

Tg 008 Southern Blotting

for a final conc. of 100μg Salmon Sperm DNA/1 ml hybridization solution. Stock solution is 10 mg/ml. 15. Make a hole with a syringe needle in top of tube to release excess pressure and boil the probe for 10 minutes in the heating block must be kept

Small scale DNA preps for Neurospora crassa.

to remove most of the supernatant. 3. Add approximately 150 microliters of Ottawa Sand (Fisher Scientific # S-23) plus 500 microliters of isolation buffer (50 mM Tris-HCl pH 8, 170 mM EDTA pH 8, 1 N-lauroylsarcosine) to each tube and vortex

Small scale DNA preps for Neurospora crassa.

in a microcentrifuge. 1. Inoculate 500 microliters of liquid growth medium (Vogel's, 1.5% sucrose) in 1.5 ml microcentrifuge tubes that have had an air hole poked in the top of each with a 20 gauge needle. Shake for one to two days at 34 degrees C